TransScript? Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)
耐高溫全預(yù)混第一鏈cDNA合成試劑盒(一步法去除gDNA)(用于qPCR)
目錄號(hào): AU341-02
單 價(jià):¥6110
產(chǎn)品詳情介紹
TransScript? Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)包含反轉(zhuǎn)錄反應(yīng)所需的全部成分(TransScript? Uni RT, RNase Inhibitor, Anchored Oligo(dT)20 Primer, Random Primer(N9), dNTPs, Buffer),可在同一管反應(yīng)中實(shí)現(xiàn)cDNA 合成和去除基因組DNA。反應(yīng)時(shí),只需加入5×All-in-One Reaction Mix for qPCR、TransScript? Uni All-in-One Enzyme Mix、模板RNA和水,在42℃-65℃條件下即可高效地合成第一鏈cDNA,同時(shí)去除RNA模板中殘留的基因組DNA 。另配有20×No RT Control Mix,用于配制無(wú)反轉(zhuǎn)錄酶的對(duì)照,判斷qPCR模板是否來(lái)自cDNA。該產(chǎn)品操作簡(jiǎn)便,降低操作過(guò)程中的污染機(jī)率。cDNA產(chǎn)物只適用于qPCR,不適用于常規(guī)PCR。

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