TransScript? Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)
耐高溫全預(yù)混第一鏈cDNA合成試劑盒（一步法去除gDNA）（用于qPCR）
| 目錄號 | 規(guī)格 | 單價(jià) |
|---|---|---|
| AU341-02 | 100 rxns | 6110 |
產(chǎn)品詳情介紹
TransScript? Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)包含反轉(zhuǎn)錄反應(yīng)所需的全部成分(TransScript? Uni RT, RNase Inhibitor, Anchored Oligo(dT)20 Primer, Random Primer(N9), dNTPs, Buffer)，可在同一管反應(yīng)中實(shí)現(xiàn)cDNA 合成和去除基因組DNA。反應(yīng)時(shí)，只需加入5×All-in-One Reaction Mix for qPCR、TransScript? Uni All-in-One Enzyme Mix、模板RNA和水，在42℃-65℃條件下即可高效地合成第一鏈cDNA，同時(shí)去除RNA模板中殘留的基因組DNA 。另配有20×No RT Control Mix，用于配制無反轉(zhuǎn)錄酶的對照，判斷qPCR模板是否來自cDNA。該產(chǎn)品操作簡便，降低操作過程中的污染機(jī)率。cDNA產(chǎn)物只適用于qPCR，不適用于常規(guī)PCR。
1.Qin T, Zhang G, Zheng Y, et al. A population of stem cells with strong regenerative potential discovered in deer antlers[J]. Science, 2023.(IF 56.90)
2.Fan T, Xiao C, Liu H, et al. CXXC finger protein 1 (CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma[J]. Signal Transduction and Targeted Therapy, 2023.(if 39.30)
3.Li X, Zhang K, Yang X, et al. The cytokine CSBF inhibits the IL-17A and TNF-α inflammatory pathways via SUSD2-ACT1 in keratinocytes and alleviates IMQ-induced psoriasis[J]. Cellular & Molecular Immunology, 2025.(IF 19.80)
4.Ding X, Xing X, Liu J, et al. Cobweb‐Inspired Microenvironment‐Targeting Nanosystem with Sequential Multiple‐Stage Stimulus‐Response Capacity for Ischaemic Tissue Repair[J]. Advanced Functional Materials, 2023.(IF 19.00)
5.Chen N, Guo L, Wang L, et al. Sleep fragmentation exacerbates myocardial ischemia?reperfusion injury by promoting copper overload in cardiomyocytes[J]. Nature Communications, 2024.(IF 14.70)
6.He L, Ma S, Ding Z, et al. Inhibition of NFAT5‐Dependent Astrocyte Swelling Alleviates Neuropathic Pain[J]. Advanced Science, 2024.(IF 14.30)
7.Li C, Tan Y P, Gao D, et al. IRE1α RNase activity is critical for early embryo development by degrading maternal transcripts[J]. Nucleic Acids Research, 2025.(IF 13.10)
8.Li M, Hu Z, Huang Y, et al. BiFC and FACS-based CRISPR screening revealed that QKI promotes PABPN1 LLPS in colorectal cancer cells[J]. Protein & Cell, 2025.(IF 12.80)
9.Kong X, Tan S, Guan M, et al. Nanocarrier-mediated transdermal delivery of Lmidgf4 dsRNA expedites biological control of locusts by Beauveria bassiana[J]. Journal of Nanobiotechnology, 2025.(IF 12.60)
10.Wei B, Liu Y, Lyu M, et al. Natural variation in CHELATASE SUBUNIT IA increases grain weight and enhances wheat yield[J]. The Plant Cell, 2025.(IF11.60)
11.Cheng H, Liu S, Zhang Y, et al. Comparative single-cell transcriptomic map reveals divergence in leaves between two cotton species at cell type resolution[J]. Journal of Advanced Research, 2025.(IF 11.40)
12.Liao R, Wu Y, Qin L, et al. BCL11B and the NuRD complex cooperatively guard T‐cell fate and inhibit OPA1‐mediated mitochondrial fusion in T cells[J]. The EMBO Journal, 2023.(IF 11.40)
13.Liang X, Liu J, Liu X, et al. LINP1 represses unfolded protein response by directly inhibiting eIF2α phosphorylation to promote cutaneous squamous cell carcinoma[J]. Experimental Hematology & Oncology, 2023.(IF 10.90)
14.Yang S, Li W, Bai X, et al. Ginseng-derived nanoparticles alleviate inflammatory bowel disease via the TLR4/MAPK and p62/Nrf2/Keap1 pathways[J]. Journal of Nanobiotechnology, 2024.(IF 10.60)
15.Jin Y, Liu X, Liang X, et al. Resveratrol rescues cutaneous radiation-induced DNA damage via a novel AMPK/SIRT7/HMGB1 regulatory axis[J]. Cell Death & Disease, 2022.(IF 9.69)
16.Li C, Wang K, Zhao L, et al. LINC00622 transcriptionally promotes RRAGD to repress mTORC1-modulated autophagic cell death by associating with BTF3 in cutaneous melanoma[J]. Cell Death & Disease, 2025.(IF 9.60)
17.Li Q, Wang R, Xue J, et al. ZIF-8-modified black phosphorus nanosheets incorporated into injectable dual-component hydrogels for enhanced photothermal antibacterial and osteogenic activities[J]. ACS Applied Materials & Interfaces, 2024.(IF 8.50)
18.Luo Z, Guo J, Pang S, et al. Discovery of FO-4-15, a novel 1, 2, 4-oxadiazole derivative, ameliorates cognitive impairments in 3× Tg mice by activating the mGluR1/CaMKIIα pathway[J]. Acta Pharmacologica Sinica, 2025.(IF 8.40)
19.Lei H, Guo K, Shu G, et al. GJB2 as a novel prognostic biomarker associated with immune infiltration and cuproptosis in ovarian cancer[J]. Apoptosis, 2025.(IF 8.10)
20.Yuan Y, Fu D, Xu Y, et al. Pt (IV) Prodrug as a Potential Antitumor Agent with APE1 Inhibitory Activity[J]. Journal of Medicinal Chemistry, 2022.(IF 8.04)
21.Sun Y, Zhang R, Li J, et al. 2-Ethylhexyl diphenyl phosphate induces lung oxidative stress and pyroptosis in chicks[J]. Science of The Total Environment, 2025.(IF 8.00)
22.Yan J, Zhang P, Liu X, et al. Obesity modulates hematopoietic stem cell fate decision via IL-1β induced p38/MAPK signaling pathway[J]. Stem Cell Research & Therapy, 2024.(IF 7.10)
23.Liu X, Zhang H, Yan J, et al. Deciphering the efficacy and mechanism of astragalus membranaceus on high altitude polycythemia by integrating network pharmacology and in vivo experiments[J]. Nutrients, 2022.(IF 6.71)
24.Yu W, Wang Y, Jiang D, et al. A saponin from astragalus promotes pancreatic ductal organoids differentiation into insulin-producing cells[J]. Phytomedicine, 2022.(IF 6.66)
25.Xia Y, Chen K, Wang Y, et al. Importance of Selenoprotein O in Regulating Hmgb1: A New Direction for Modulating ROS-Dependent NETs Formation to Aggravate the Progression of Acute Liver Inflammation[J]. Journal of Agricultural and Food Chemistry, 2025.(IF 6.20)
26.Chen L, Ma S, Cao A, et al. Bile acids promote lipopolysaccharide clearance via the hepato-biliary pathway in broiler chickens[J]. Ecotoxicology and Environmental Safety, 2024.(IF 6.20)
27.Xia Y, Luo D, Xu A, et al. Insight into the mechanism of melatonin in attenuating PCB126-induced liver injury: Resistance to ROS-dependent NETs formation to alleviate inflammation and lipid metabolism dysfunction[J]. Ecotoxicology and Environmental Safety, 2024.(IF 6.20)
28.Yang L, Wu X, Liu S, et al. Comprehensive Analysis of BrHMPs Reveals Potential Roles in Abiotic Stress Tolerance and Pollen–Stigma Interaction in Brassica rapa[J]. Cells, 2023.(IF 6.00)
29.Du J, Li Z, Cao X, et al. Mechanism of mitochondrial kinetic imbalance and Nrf2 signaling pathway-mediated oxidative stress in nickel and/or chromium-induced kidney injury in mice[J]. Antioxidants, 2024.(IF 6.00)
30.Zhang W, Zhang R, Chang Z, et al. Resveratrol activates CD8+ T cells through IL-18 bystander activation in lung adenocarcinoma[J]. Frontiers in Pharmacology, 2022.(IF 5.99)
31.Liu Y, Wang W, Cao Y, et al. Selenium‐Chitosan Reduces Postharvest Decay of Red Globe Grapes by Improving Disease Resistance During Cold Storage[J]. eFood, 2025.(IF 5.70)
32.Xu W, Sang S, Wang J, et al. Identification of telomere-related lncRNAs and immunological analysis in ovarian cancer[J]. Frontiers in Immunology, 2024.(IF 5.70)
33.Guo S, Zhong A, Zhang D, et al. ATP2B3 Inhibition Alleviates Erastin–Induced Ferroptosis in HT-22 Cells through the P62–KEAP1–NRF2–HO-1 Pathway[J]. International Journal of Molecular Sciences, 2023.(IF 5.60)
34.Jing-Lun Z, Shuang C, Li-Mei Z, et al. YKL-40 promotes chemokine expression following drug-induced liver injury via TF-PAR1 pathway in mice[J]. Frontiers in pharmacology, 2023.(IF 5.60)
35.Xing X, Hu H, Hu H, et al. Quercetin from Penthorum chinense Pursh protects against LPS-induced liver inflammation in chickens via suppressing pyroptosis-macrophage polarization crosstalk[J]. Journal of Ethnopharmacology, 2025.(IF 5.40)
36.Xu P, Zhao Y, Feng Y, et al. Deoxynivalenol induces m6A-mediated upregulation of p21 and growth arrest of mouse hippocampal neuron cells in vitro[J]. Cell Biology and Toxicology, 2024.(IF 5.30)
37.Wang L, Zhang J, Qiu S, et al. IL-33/ST2 drives inflammatory pain via CCL2 signaling and activation of TRPV1 and TRPM8[J]. Communications Biology, 2025.(IF 5.10)
