TransStart? FastPfu Fly DNA Polymerase
FastPfu Fly 快速高保真DNA 聚合酶
| 目錄號 | 規(guī)格 | 單價 | 
|---|---|---|
| AP231-21 | 250 units | 770 | 
| AP231-22 | 500 units | 1390 | 
| AP231-23 | 6×500 units | 7060 | 
產(chǎn)品詳情介紹
TransStart?FastPfu Fly DNA Polymerase是用于快速PCR的熱啟動超高保真DNA聚合酶。該酶與TransStart? FastPfu DNA Polymerase相比,擴增速度更快(≤5 kb基因可實現(xiàn)12 kb/min的極速擴增,>5 kb基因可達到6 kb/min高速擴增),擴增效率更高,產(chǎn)量更高,保真性更高,靈敏度更高。2×TransStart? FastPfu Fly Reaction Mix中已含有dNTPs,DNA擴增時,只需加入模板、引物、水和TransStart? FastPfu Fly DNA Polymerase,使Reaction Mix的濃度為1×即可進行反應。擴增產(chǎn)物為平末端,可直接克隆于pEASY?-Blunt系列載體中。
? 保真性是EasyTaq? DNA Polymerase的108倍。
? 擴增產(chǎn)物為平末端,可直接克隆于pEASY?-Blunt系列載體中。
? 基因組DNA片段的擴增(≤15 kb)。
? Plasmid DNA片段擴增(≤ 20 kb)。


使用TransGen、Company TA及Company N產(chǎn)品,分別以人gDNA為模板,進行不同基因的PCR擴增,1.0%瓊脂糖凝膠電泳分析擴增效果。
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