TransStart? FastPfu DNA Polymerase (含 2.5 mM dNTPs)
FastPfu快速高保真DNA 聚合酶(含 2.5 mM dNTPs)
| 目錄號(hào) | 規(guī)格 | 單價(jià) | 
|---|---|---|
| AP221-11 | 250 units | 630 | 
| AP221-12 | 500 units | 1130 | 
| AP221-13 | 6×500 units | 5750 | 
產(chǎn)品詳情介紹
TransStart? FastPfu DNA Polymerase 是用于快速PCR的熱啟動(dòng)高保真DNA聚合酶,TransStart? FastPfu DNA Polymerase擴(kuò)增效率高、擴(kuò)增速度快(4 kb/min,是普通Pfu酶的8倍),克服了普通Pfu酶擴(kuò)增效率低、產(chǎn)量低和擴(kuò)增速度慢(0.5 kb/min)的缺陷,極大地縮短了反應(yīng)時(shí)間。
· 保真性是EasyTaq? DNA Polymerase的54倍。
· 擴(kuò)增產(chǎn)物為平端,可直接克隆于pEASY?-Blunt系列載體中。
· 基因組DNA片段的擴(kuò)增 (≤ 15 kb)。
· Plasmid DNA片段的擴(kuò)增 (≤ 20 kb)。



1.Lei Z, Meng H, Liu L, et al. Mitochondrial base editor induces substantial nuclear off-target mutations[J]. Nature, 2022.(IF 69.50)
2.Zhang H, Zhu Y, Liu Z, et al. A volatile from the skin microbiota of flavivirus-infected hosts promotes mosquito attractiveness[J]. Cell, 2022.(IF 66.85)
3.Wang Y, Zhang S, Yang X, et al. Mesoscale DNA feature in antibody-coding sequence facilitates somatic hypermutation[J]. Cell, 2023.(IF 64.50)
4.Lin Q, Jin S, Zong Y, et al. High-efficiency prime editing with optimized, paired pegRNAs in plants[J]. Nature Biotechnology, 2021.(IF 54.90)
5.Zong Y, Liu Y, Xue C, et al. An engineered prime editor with enhanced editing efficiency in plants[J]. Nature Biotechnology, 2022.(IF 54.00)
6.Liu J L, Yan X Q, Wu H, et al. RNA codon expansion via programmable pseudouridine editing and decoding[J]. Nature, 2025.(IF 48.50)
7.Yang J, Zhao T, Fan J, et al. Structure-guided discovery of bile acid derivatives for treating liver diseases without causing itch[J]. Cell, 2024.(IF 45.60)
8.Jin S, Lin Q, Luo Y, et al. Genome-wide specificity of prime editors in plants[J]. Nature Biotechnology, 2021.(IF 36.55)
9.Wang S, Zong Y, Lin Q, et al. Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9[J]. Nature biotechnology, 2020.(IF 36.55)
10.Li C, Zhang R, Meng X, et al. Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors[J]. Nature biotechnology, 2020.(IF 35.72)
11.Zong Y, Liu Y, Xue C, et al. An engineered prime editor with enhanced editing efficiency in plants[J]. Nature Biotechnology, 2022.(IF 54.00)
12.Lin Q, Zong Y, Xue C, et al. Prime genome editing in rice and wheat[J]. Nature biotechnology, 2020.(IF 31.90)
13.Song B, Chen Y, Liu X, et al. Ordered assembly of the cytosolic RNA-sensing MDA5-MAVS signaling complex via binding to unanchored K63-linked poly-ubiquitin chains[J]. Immunity, 2021.(IF 31.75)
14.Li Y, Zhao L, Zhang Y, et al. Structural basis for product specificities of MLL family methyltransferases[J]. Molecular Cell, 2022.(IF 19.33)
15.Li C, Zong Y, Jin S, et al. SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds[J]. Genome biology, 2020.(IF 18.36)
16.Zhang H, Li Z, Zhou S, et al. A fungal NRPS-PKS enzyme catalyses the formation of the flavonoid naringenin[J]. Nature Communications, 2022.(IF 17.69)
17.Lei Y, Fei P, Song B, et al. A loosened gating mechanism of RIG-I leads to autoimmune disorders[J]. Nucleic acids research, 2022.(IF 16.97)
18.Song Z, Zhou S, Zhang H, et al. Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex[J]. Nature Communications, 2023.(IF 16.60)
19.Fan J, Ran H, Wei P L, et al. An ortho‐Quinone Methide Mediates Disulfide Migration in the Biosynthesis of Epidithiodiketopiperazines[J]. Angewandte Chemie International Edition, 2023.(IF 16.82)
20.Fan J, Ran H, Wei P L, et al. Pretrichodermamide A Biosynthesis Reveals the Hidden Diversity of Epidithiodiketopiperazines[J]. Angewandte Chemie, 2023.(IF 16.82)
21.Jin S, Fei H, Zhu Z, et al. Rationally designed APOBEC3B cytosine base editors with improved specificity[J]. Molecular cell, 2020.(IF 15.58)
22.Lin R, Liang J, Wang R, et al. The raphe dopamine system controls the expression of incentive memory[J]. Neuron, 2020.(IF 14.40)
23Bi L, Qin Z, Wang T, et al. The convergence of head-on DNA unwinding forks induces helicase oligomerization and activity transition[J]. Proceedings of the National Academy of Sciences, 2022.(IF 12.78)
24.Ni P, Zhao Y, Zhou X, et al. Efficient and versatile multiplex prime editing in hexaploid wheat[J]. Genome Biology, 2023.(IF 12.30)
25.Chen G, Wang D, Wu B, et al. Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation[J]. Nature communications, 2020.(IF 12.12)
26.Liu X, Guo X, Niu L, et al. Atlastin-1 regulates morphology and function of endoplasmic reticulum in dendrites[J]. Nature communications, 2019.(IF 11.88)
27.Zhu C, Li X, Zhang M, et al. ERF. D2 negatively controls drought tolerance through synergistic regulation of abscisic acid and jasmonic acid in tomato[J]. Plant Biotechnology Journal, 2025.(IF 11.20)
28.Chen L, Huang X X, Zhao S M, et al. IPyA glucosylation mediates light and temperature signaling to regulate auxin-dependent hypocotyl elongation in Arabidopsis[J]. Proceedings of the National Academy of Sciences, 2020.(if 10.60)
29.Jin S, Gao Q, Gao C. An unbiased method for evaluating the genome-wide specificity of base editors in rice[J]. Nature Protocols, 2021.(IF 10.40)



 
             
                     
                 
		   
							 
							 
							 
							 
							 
							 
							 
							 
							 
							 
							
 
             
                    
 
                    
