產(chǎn)品詳情介紹
本產(chǎn)品利用特殊的重組酶和同源重組的原理,可以將任意方法線性化后的載體和與其兩端具有15-25 bp重疊區(qū)域的PCR片段定向重組,可以實(shí)現(xiàn)1-7個(gè)片段的高效無(wú)縫拼接。
? 快速:僅需要5~15分鐘反應(yīng)時(shí)間。
? 簡(jiǎn)單:不受片段酶切位點(diǎn)的影響,無(wú)需對(duì)片段酶切。
? 高效:陽(yáng)性率達(dá)95% 以上。
? 無(wú)縫:不引入額外的序列。
1 Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 38.07)
2 Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022(IF 14.90)
3 Jin Q, Yang X, Gou S, et al. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression[J]. Science China Life Sciences, 2022.(IF 10.37)
4 Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022.(IF 63.71)
5 Li B, Zhu L, Lu C, et al. circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity[J]. Nature communications, 2021.(IF 12.12)
6 Liu S, Fan L, Liu Z, et al. A Pd1–Ps–P1 feedback loop controls pubescence density in soybean[J]. Molecular plant, 2020.(IF 12.08)